Article, system, and method for indication of treatment

ABSTRACT

An article, a system, and a method for indication of treatment are provided. The article comprises a first body, a second body, and a treatment indicator. The first body comprises a first axis, a cavity, a first port, and a second port. The cavity is positioned within the first body and configured to receive the second body. The second body comprises a second axis, a chamber, a third port, and a fourth port. The article is configured to move between a first configuration and a second configuration. In the first configuration, the first port is aligned with the third port to form a fluid pathway to the chamber, and the second port is aligned with the fourth port to form a fluid pathway to the chamber. In the second configuration, the first port is misaligned with the third port, and the second port is misaligned with the fourth port.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application claims priority under 35 U.S.C. § 120 toco-pending U.S. provisional Patent Application Ser. No. 62/785,971,filed Dec. 28, 2018 and to co-pending U.S. provisional PatentApplication Ser. No. 62/786,075, filed Dec. 28, 2018, each of which isincorporated by reference herein in its entirety.

The present application is related to co-pending application entitled “ATREATMENT INDICATOR, A METHOD OF PRODUCTION THEREOF, AND A METHOD OF USETHEREOF” Attorney Docket No. 180473/ASP6001USNP1 filed on the same dateas the present disclosure and incorporated by reference herein in itsentirety.

FIELD

The present disclosure relates to an article, a system, and a method forindication of treatment.

BACKGROUND

Various medical devices are used in numerous procedures in the medicalfield. These devices are as varied as the procedures themselves. After amedical device, such as an endoscope, is used, the medical device istreated (e.g., cleaned, disinfected, and/or sterilized) in order toprepare the medical device for its next use. Ensuring the medical deviceis properly treated prior to the ultimate use on a patient can preventthe risk of cross contamination and the spread of disease.

SUMMARY

The present disclosure provides an article for indication of treatment.The article comprises a first body, a second body, and a treatmentindicator. The first body comprises a first axis, a cavity, a firstport, and a second port. The cavity is positioned within the first bodyand configured to receive the second body. The second port is offsetfrom the first port relative to the first axis. The second bodycomprises a second axis, a chamber, a third port, and a fourth port. Thechamber is positioned within the second body and configured to receive afluid. The fourth port is offset from the third port relative to thesecond axis. The article is configured to move between a firstconfiguration and a second configuration. In the first configuration,the first port is aligned with the third port to form a fluid pathway tothe chamber, and the second port is aligned with the fourth port to forma fluid pathway to the chamber. In the second configuration, the firstport is misaligned with the third port, and the second port ismisaligned with the fourth port. The treatment indicator is positionedwithin the chamber.

The present disclosure also provides a system comprising an article forindication of a treatment and a detector. The article comprises a firstbody, a second body, and a treatment indicator. The first body comprisesa first axis, a cavity, a first port, and a second port. The cavity ispositioned within the first body and configured to receive the secondbody. The second port is offset from the first port relative to thefirst axis. The second body comprises a second axis, a chamber, a thirdport, a fourth port, and a transparent section. The chamber ispositioned within the second body and configured to receive a fluid. Thefourth port is offset from the third port relative to the second axis.The transparent section is configured to transmit electromagneticradiation through the chamber. The article is configured to move betweena first configuration and a second configuration. In the firstconfiguration, the first port is aligned with the third port to form afluid pathway to the chamber, and the second port is aligned with thefourth port to form a fluid pathway to the chamber. In the secondconfiguration, the first port is misaligned with the third port, and thesecond port is misaligned with the fourth port. The treatment indicatoris positioned within the chamber. The detector is configured to measurean electromagnetic property of fluid within the chamber via thetransparent section of the second body.

The present disclosure also provides a system comprising an apparatusfor treatment and an article for indication of treatment. The apparatusfor treatment comprises a treatment basin and a vessel. The treatmentbasin is configured to receive a device to be treated. The vessel ispositioned separate from the treatment basin and is in fluidcommunication with the treatment basin. The vessel is suitable toreceive the article for indication of treatment and to provide atreatment agent to the article. The article comprises a first body, asecond body, and a treatment indicator. The first body comprises a firstaxis, a cavity, a first port, and a second port. The cavity ispositioned within the first body and configured to receive the secondbody. The second port is offset from the first port relative to thefirst axis. The second body comprises a second axis, a chamber, a thirdport, and a fourth port. The chamber is positioned within the secondbody and configured to receive a fluid. The fourth port is offset fromthe third port relative to the second axis. The article is configured tomove between a first configuration and a second configuration. In thefirst configuration, the first port is aligned with the third port toform a fluid pathway to the chamber, and the second port is aligned withthe fourth port to form a fluid pathway to the chamber. In the secondconfiguration, the first port is misaligned with the third port, and thesecond port is misaligned with the fourth port. The treatment indicatoris positioned within the chamber.

In a further example, the present disclosure also provides an articlefor indication of treatment. The article comprises a first body, asecond body, a treatment indicator, and a reservoir. The first bodycomprises a first axis, a cavity, a first port, and a second port. Thecavity is positioned within the first body and configured to receive thesecond body. The second port is offset from the first port relative tothe first axis. The second body comprises a second axis, a chamber, athird port, a fourth port, and a transparent section. The chamber ispositioned within the second body and configured to receive a fluid. Thefourth port is offset from the third port relative to the second axis.The transparent section is configured to transmit electromagneticradiation through the chamber. The article is configured to move betweena first configuration and a second configuration. In the firstconfiguration, the first port is aligned with the third port to form afluid pathway to the chamber, and the second port is aligned with thefourth port to form a fluid pathway to the chamber. In the secondconfiguration, the first port is misaligned with the third port, and thesecond port is misaligned with the fourth port. The treatment indicatoris positioned within the chamber and comprises biological material. Thereservoir is positioned within the chamber and comprises a sealconfigured to retain growth media separate from the treatment indicator.The seal is configured to break responsive to the second configurationof the article. Breaking of the seal is suitable to introduce the growthmedia to the treatment indicator.

The present disclosure also provides a method for indication oftreatment. The method comprises introducing an article for treatmentinto an apparatus for treatment. The article comprises a first body, asecond body, a treatment indicator, and a reservoir. The first bodycomprises a first axis, a cavity, a first port, and a second port. Thecavity is positioned within the first body and configured to receive thesecond body. The second port is offset from the first port relative tothe first axis. The second body comprises a second axis, a chamber, athird port, a fourth port, and a transparent section. The chamber ispositioned within the second body and configured to receive a fluid. Thefourth port is offset from the third port relative to the second axis.The transparent section is configured to transmit electromagneticradiation through the chamber. The article is configured to move betweena first configuration and a second configuration. In the firstconfiguration, the first port is aligned with the third port to form afluid pathway to the chamber, and the second port is aligned with thefourth port to form a fluid pathway to the chamber. In the secondconfiguration, the first port is misaligned with the third port, and thesecond port is misaligned with the fourth port. The treatment indicatoris positioned within the chamber and comprises biological material. Thereservoir is positioned within the chamber and comprises a sealconfigured to retain growth media separate from the treatment indicator.The seal is configured to break responsive to the second configurationof the article. Breaking of the seal is suitable to introduce the growthmedia to the treatment indicator. A treatment solution is introducedinto the chamber of the article in the first configuration. Thetreatment solution is removed from the chamber. The article is changedfrom the first configuration to the second configuration to contact thetreatment indicator with the growth media. An electromagnetic propertyof at least one of the growth media and treatment indicator is measured.

It is understood that the inventions described in this specification arenot limited to the examples summarized in this Summary. Various otherexamples are described and exemplified herein.

BRIEF DESCRIPTION OF THE DRAWINGS

The features and advantages of the examples, and the manner of attainingthem, will become more apparent, and the examples will be betterunderstood by reference to the following description taken inconjunction with the accompanying drawings, wherein:

FIG. 1A is a perspective view illustrating one example of an article forindication of treatment according to the present disclosure;

FIG. 1B is a perspective exploded view of the article for indication oftreatment of FIG. 1A;

FIG. 2A is a front-elevational view of an article for indication oftreatment of FIG. 1 in a first configuration;

FIG. 2B is a front-elevational view of the article for indication oftreatment of FIG. 1 in a second configuration;

FIG. 3A is a perspective view illustrating another example of an articlefor indication of treatment according to the present disclosure;

FIG. 3B is a front-elevational view of the article for indication oftreatment of FIG. 3A;

FIG. 3C is a perspective exploded view of the article for indication oftreatment of FIG. 3A;

FIG. 4A is a cross-sectional view of the article for indication of FIG.3A in a first configuration;

FIG. 4B is a cross-sectional view of the article for indication of FIG.3A in a second configuration;

FIG. 5 is an example of a system comprising an article for indication oftreatment and a detector according to the present disclosure; and

FIG. 6 is an example of a system comprising an apparatus for treatmentand an article for indication of treatment according to the presentdisclosure.

Corresponding reference characters indicate corresponding partsthroughout the several views. The exemplifications set out hereinillustrate certain examples in one form, and such examples are not to beconstrued as limiting the scope of the present disclosure in any manner.

DETAILED DESCRIPTION

Certain examples of the present disclosure will now be described toprovide an overall understanding of the principles of the structure,function, manufacture, and use of the devices and methods disclosedherein. Various examples are illustrated in the accompanying drawings.Those of ordinary skill in the art will understand that the devices andmethods specifically described herein and illustrated in theaccompanying drawings are non-limiting examples and that the scope ofthe various examples of the present disclosure is defined solely by theclaims.

Reference throughout the specification to “various examples,” “someexamples,” “one example,” “an example,” or the like, means that aparticular feature, structure, or characteristic described in connectionwith the example is included in at least one example. Thus, appearancesof the phrases “in various examples,” “in some examples,” “in oneexample”, “in an example,” or the like, in places throughout thespecification are not necessarily all referring to the same example.Furthermore, the particular features, structures, or characteristics maybe combined in any suitable manner in one example or two or moreexamples. Thus, the particular features, structures, or characteristicsillustrated or described in connection with one example may be combined,in whole or in part, with the features, structures, or characteristicsof one other example or two or more examples without limitation. Suchmodifications and variations are intended to be included within thescope of the present disclosure.

In the present disclosure, unless otherwise indicated, all numericalparameters are to be understood as being prefaced and modified in allinstances by the term “about,” in which the numerical parameters possessthe inherent variability characteristic of the underlying measurementtechniques used to determine the numerical value of the parameter. Atthe very least, and not as an attempt to limit the application of thedoctrine of equivalents to the scope of the claims, each numericalparameter described herein should at least be construed in light of thenumber of reported significant digits and by applying ordinary roundingtechniques.

Also, any numerical range recited herein includes all sub-rangessubsumed within the recited range. For example, a range of “1 to 10”includes all sub-ranges between (and including) the recited minimumvalue of 1 and the recited maximum value of 10, that is, having aminimum value equal to or greater than 1 and a maximum value equal to orless than 10. Any maximum numerical limitation recited in thisspecification is intended to include all lower numerical limitationssubsumed therein, and any minimum numerical limitation recited in thisspecification is intended to include all higher numerical limitationssubsumed therein. Accordingly, Applicant reserves the right to amendthis specification, including the claims, to expressly recite anysub-range subsumed within the ranges expressly recited. All such rangesare inherently described in this specification such that amending toexpressly recite any such sub-ranges would comply with the requirementsof 35 U.S.C. § 112 and 35 U.S.C. § 132(a).

The grammatical articles “a,” “an,” and “the,” as used herein, areintended to include “at least one” or “one or more,” unless otherwiseindicated, even if “at least one” or “one or more” is expressly used incertain instances. Thus, the articles are used herein to refer to one ormore than one (i.e., to “at least one”) of the grammatical objects ofthe article. Further, the use of a singular noun includes the plural,and the use of a plural noun includes the singular, unless the contextof the usage requires otherwise.

A device such as a medical device (e.g., and endoscope) can undergo atreatment process to prevent cross-contamination and the spread ofdisease. As used herein, a “treatment process” may be a cleaningprocess, a disinfecting process, a sterilization process, the like, andcombinations thereof. A treatment process may be either manual,automated, or some combination thereof, and may utilize a treatmentagent. As used herein, a “treatment agent” can comprise at least one ofa cleaning agent, a disinfectant, and a sterilant. As used herein a“cleaning process” means a treatment process employing a cleaning agentthat removes and/or eliminates debris such as, for example, a dirt, adust, a particle, an oil, a protein, a carbohydrate, and the like. Asused herein, a “cleaning agent” means a type of treatment agent thatremoves and/or eliminates debris during a cleaning process such as, forexample, a surfactant and/or a detergent.

A disinfecting process and a sterilization process can remove and/oreliminate a bioburden from a device. A bioburden may be, for example, abacterium (e.g., mycobacterium, bacterial spores), an archaeon, aeukaryote, a virus, a fungus, and/or other forms of biological agents.Bacterial spores (e.g., endospores) are a form of bacteria which aredormant and highly resistive to physical and chemical degradation. Asused herein, a “disinfecting process” means a treatment process thatsubstantially removes a bioburden except for bacterial spores. As usedherein, “substantially remove” means that at least 99% of the bioburdenhas been removed from the device such as, for example, at least 99.9% ofthe bioburden, at least 99.99% of the bioburden, at least 99.999% of thebioburden, or at least 99.9999% of the bioburden has been removed fromthe device. As used herein, a “sterilization process” means a treatmentprocess which substantially removes a bioburden including bacterialspores. The sterilization process may include, for example, the additionof heat, freezing, a sterilant, irradiation, pressure, and combinationsthereof. The sterilant may comprise a chemical capable of sterilization.The disinfection process may include, for example, the addition of heat,a disinfectant, irradiation, pressure, and combinations thereof. Thedisinfectant may comprise a chemical capable of disinfection.

Ensuring that a treatment process is properly performed on a device cansupport prevention of cross-contamination and the spread of disease.Testing a device, such as a medical device (e.g., an endoscope),directly may not be practical due to cost and/or time constraints. Thus,the present disclosure provides an article, a method, and a system forindication of treatment that can be employed to determine theeffectiveness of a treatment process to which the device was subjected.

FIGS. 1A-B and 2A-B illustrate one example of an article 100 forindication of treatment according to the present disclosure. The article100 can comprise a first body 102 and a second body 104. The first body102 may comprise a cavity 108 positioned therein and configured toreceive at least a portion of the second body 104. The cavity 108 may beany size and shape that allows for cooperative engagement with thesecond body 104. The first body 102 may cooperatively engage the secondbody 104 in a partially or fully sealed orientation. As illustrated, thefirst body 102 may be a sleeve-like I-shaped member, and the second body104 may comprise a generally rectangular chamber 116 that can bereceived into the cavity 108 of the first body 102. An axis 106 extendsthrough the first body 102 from a first end 102 a to a second end 102 band illustrates one longitudinal orientation by which the second body104 may engage the first body 102. The second end 102 b can beoppositely disposed from the first end 102 a.

The first body 102 can comprise at least two ports 110 and 112. Eachport 110, 112 can be a bore through the first body 102. The ports 110and 112 can be suitable to receive fluid and transport fluidtherethrough. Port 112 can be offset from port 110 relative to axis 106,as illustrated. For example, port 112 can be more proximal to the secondend 102 b of the first body 102 than port 110. Port 112 can be adjacentto the second end 102 b. Port 112 can also be radially offset from port110 relative to axis 106.

The second body 104 can be positioned within the cavity 108 of the firstbody 102. The second body 104 can comprise an axis 114. The second body104 can further comprise a chamber 116 configured to receive a fluid.Axis 114 can extend from a first end 104 a of the second body 104 to asecond end 104 b of the second body 104. Axis 114 can be the same asaxis 106. The second end 104 b of the second body 104 can be oppositelydisposed from the first end 104 a. When the first body 102 and thesecond body 104 are engaged, the first end 102 a of the first body 102can be adjacent to the first end 104 a of the second body 104 and thesecond end 102 b of the first body 102 can be adjacent to the second end104 b of the second body 104.

The second body 104 can comprise at least two ports 118 and 120 that,for example, pair with ports 110, 112. Each port 118, 120 can be a borethrough the second body 104. Port 118 and port 120 can be suitablyconfigured to receive fluid and transport fluid therethrough. Port 120can be offset from port 118 relative to axis 114, as illustrated. Forexample, port 120 can be more proximal to the second end 104 b than port118. Port 120 can be adjacent to the second end 104 b. Port 102 can alsobe radially offset from port 118 relative to axis 114.

The ports 110, 112, 118, 120 can comprise any shape suitably configuredto receive and transport fluid. For example, the ports 110, 112, 118,120 can comprise at least one of a generally circular, a generallyrectangular, and a generally oval cross-sectional shape.

A treatment indicator 122 can be positioned within the chamber 116 andcan comprise biological material. For example, the biological materialcan comprise at least one of an archaeon, an eukaryote, a bacterium(e.g., bacterial spore), and a virus. The biological material degradesresponsive to contact with at least one of a disinfectant and asterilant. The level of degradation of the biological material in thetreatment process can be dependent on, for example, at least one of thetype of disinfectant and/or sterilant used, the contact time between thedisinfectant and/or sterilant and the biological material, thetemperature of the disinfectant and/or sterilant, and the activity ofthe disinfectant and/or sterilant.

The biological material can multiply (e.g., reproduce) responsive tocontact with a growth media. The rate and/or amount of growth of thebiological material can provide an indication of the effectiveness of atreatment process. For example, a sterilant can degrade the biologicalmaterial on the treatment indicator 122 such that the treatmentindicator 122 is substantially free from biological material. Therefore,upon contacting the treatment indicator 122 with growth media, minimal,if any, biological material can multiply resulting in a reduced rateand/or amount of growth of the biological material compared to atreatment indicator that was not degraded by a sterilant. The reducedrate and/or amount of growth can indicate, for example, whether thetreatment process was effective or the degree to which the treatmentprocess was effective.

The growth media (e.g., culture media) can comprise various materials tosupport the growth of biological material. For example, the growth mediacan comprise at least one of agar, water, a hormone, a growth factor,blood serum, a vitamin, glucose, glycerol, an ammonium salt, nitrate,and an amino acid. The growth media can be liquid.

The second body 104 can be moveable (e.g., slidable, rotatable,telescoping, etc.) relative to the first body 102, and/or the first body102 can be moveable relative to the second body 104. For example, thearticle 100 can change position between a first configuration, asillustrated in FIG. 2A, and a second configuration, as illustrated inFIG. 2B. The configuration of the article 100 can enable or limittraversal of fluid into and/or out of the chamber 116 via, for example,the full or partial blockage of ports 110, 112, 118, 120. The fluid cancomprise at least one of growth media, a rinse agent (e.g., water,alcohol), and a treatment agent (e.g., a cleaning agent, a disinfectant,a sterilant).

An offset distance from port 120 to port 118 relative to axis 114 can bethe same as an offset distance from port 112 to port 110 relative toaxis 106 so that there is a pairing between ports 110 and 118, and ports112 and 120 when the second body 104 is received by first body 102.Movement of the first body 102 and/or second body 104 can controlcommunication between port 110 and port 118 and between port 112 andport 120 simultaneously. For example, in the first configuration, port110 can be aligned with port 118 to form a first fluid pathway 150 tothe chamber 116, and port 112 can be aligned with port 120 to form asecond fluid pathway 160 to the chamber 116. When aligned, fluid canflow into the chamber 116 utilizing ports 110, 112, 116, 118. Forexample, fluid can be introduced into port 110 and traverse through thefirst fluid pathway 150 into the chamber 116. Fluid can be introducedinto port 112 and traverse through the second fluid pathway 160 into thechamber 116. While in the chamber 116, the fluid can contact thetreatment indicator 122. In examples where the fluid comprises adisinfectant and/or sterilant, the fluid contact with the treatmentindicator 122 can degrade biological material on the treatment indicator122.

Fluid can flow out of the chamber 116 utilizing the first pathway 150and/or second fluid pathway 160. For example, fluid can be introducedinto port 118 from the chamber 116 and traverse through the first fluidpathway 150 out of the chamber 116. Fluid can be introduced into port120 from the chamber 116 and traverse through the second fluid pathway160 out of the chamber 116. The first configuration of the article 100can be suitable to gravity drain fluid from the chamber 116. Forexample, the article 100 can be oriented such that fluid in the chamber116 flows into port 120 and traverses through the second fluid pathway160 out of the chamber 116. The first fluid pathway 150 can beconfigured as an inlet, and the second fluid pathway 160 can beconfigured as an outlet. Also, for example, the second fluid pathway 160can be configured as an inlet, and the first fluid pathway 150 can beconfigured as an outlet.

The second configuration of the article 100 can inhibit and/or preventcommunication between port 110 and port 118 and between port 112 andport 120. For example, in the second configuration, port 110 can bemisaligned with port 118 and port 112 can be misaligned with port 120.The misalignment can inhibit or prevent traversal of fluid through thefirst fluid pathway 150 and/or second fluid pathway 160 via, forexample, the full or partial blockage of ports 110, 112, 118, 120. Inthe second configuration, fluid remaining within the chamber 116 may beinhibited or prevented from exiting the chamber 116. For example, in thesecond configuration, the chamber 116 may be a closed system. Growthmedia can be in the chamber 116 while the article 100 is in the secondconfiguration, and the growth media can contact the treatment indicator122.

The article 100 can change configuration by a variety of methods. Forexample, article 110 can be configured to rotate at least one of thefirst body 102 around axis 106 and the second body 104 around 114 tochange between the first configuration and the second configuration. Thearticle 100 can be configured to translate at least one of the firstbody 102 relative to axis 106, and the second body 104 relative to axis114 to change between the first configuration and the secondconfiguration.

The configuration change of the article 100 can be manual (e.g.,activated by an operator) or automatic. For example, the apparatus fortreatment can automatically change the configuration of the article 100responsive to at least a portion of a treatment process.

The article 100 can comprise additional components configured forfacilitating the configuration change of the article 100. For example,the article 100 can comprise at least one of a spring and a piston. Forexample where the article 100 comprises a spring, the spring can bepositioned between the first body 102 and the second body 104 in orderto apply a force to translate at least one of the first body 102relative to axis 106 and the second body 104 relative to axis 114.Similarly, the spring can be positioned in communication with the firstbody 102 and/or the second body 104 in order to apply a force to thefirst body 102 and/or second body 104 to rotate at least one of thefirst body 102 around axis 106 and the second body 104 around axis 114.

The treatment indicator 122 and/or fluid with the chamber 116 cancomprise an electromagnetic property. The electromagnetic property canbe, for example, at least one of absorption, transmittance, scattering,reflectance, and photoluminescence. The electromagnetic property can bemeasured in order to determine the rate and/or amount of biologicalmaterial on the treatment indicator 122. For example, electromagneticradiation can be emitted into the chamber 116, and electromagneticradiation can be detected from the chamber 116. Based on the emittedand/or detected electromagnetic radiation, the electromagnetic propertycan be determined. The electromagnetic property can correspond to a rateand/or amount of biological material on the treatment indicator 122, andthus the measured electromagnetic property can be used to determine, forexample, the rate and/or amount of biological material on the treatmentindicator 122, whether the treatment process is complete, and theeffectiveness of a treatment process. If the electromagnetic property isbelow a threshold value, the treatment process can be determined to beeffective. If the electromagnetic property is at least the thresholdvalue, the treatment process can be determined to be ineffective. Adegree of effectiveness of the treatment process can be determined basedon the measured electromagnetic property. If the treatment process isdetermined to be ineffective based on the measurement of theelectromagnetic property, the treatment process may be discontinued, orthe treatment process may continue with another treatment indicatoruntil it is later determined that the treatment process is effective,based on a subsequent reading that the electromagnetic property is belowa threshold value.

In order to facilitate a measurement of the electromagnetic property,the first body 102 can comprise an opening 140 and the second body 104can comprise a corresponding transparent section 104 c. For example, theopening 140 can be the void space created between the horizontaltop/bottom and vertical middle portion of the “I” when the first body102 is an I-shaped member, as illustrated. The transparent section 104 ccan be configured to allow transmission of electromagnetic radiationinto and/or through the chamber 116. For example, the transparentsection 104 c can comprise a material that minimally absorbs, or doesnot absorb, electromagnetic radiation that is emitted through thechamber 116. The opening 140 can be configured to align with thetransparent section 104 c of the second body 104 when the article 100 isin a second configuration as illustrated in FIG. 2B. The first body 102may be configured with a second transparent section (not shown) in placeof the opening 140 and the second transparent section can be configuredto transmit electromagnetic radiation into the transparent section 104 cthrough the chamber 116. The transmission of electromagnetic radiationinto and/or through the chamber 116 can enable measurement of anelectromagnetic property of the treatment indicator 122 and/or fluidwithin the chamber 116.

FIGS. 3A-C and 4A-B illustrate another example of an article 300 forindication of treatment according to the present disclosure is provided.The article 300 comprises a first body 302 and a second body 304. Thefirst body 302 may comprise a cavity 308 positioned therein andconfigured to receive at least a portion of the second body 304. Thecavity 308 may be any size and shape that allows for cooperativeengagement with the second body 304. The first body 302 maycooperatively engage the second body 304 in a partially or fully sealedorientation. As illustrated, the first body 302 may be a sleeve-likegenerally cylindrical-shaped member, and the second body 304 maycomprise a generally cylindrical-shaped chamber 316 that can be receivedinto the cavity 308 of the first body 302. Axis 306 extends through thefirst body 302 from a first end 302 a to a second end 302 b andillustrates one longitudinal orientation by which the second body 304may engage the first body 302. The second end 302 b can be oppositelydisposed from the first end 302 a.

The first body 302 can comprise at least four ports 310, 312, 324, and326. The ports 310, 312, 324, 326 can be suitably configured to receivefluid and transport fluid. Port 312 can be offset from port 310 relativeto axis 306. Port 324 can be offset from port 326 relative to axis 306.Port 310 can be positioned relative to axis 306 at the same position asport 324 or offset from port 324 relative to axis 306. Port 312 can bepositioned relative to axis 306 at the same position as port 326 oroffset from port 324 relative to axis 306.

The second body 304 can comprise an axis 314. Axis 314 can extend from afirst end 304 a of the second body 304 to a second end 304 b of thesecond body 304. The second end 304 b can be oppositely disposed fromthe first end 304 a. The second body can further comprise a chamber 316configured to receive a fluid.

The second body 304 can comprise at least four ports 318, 320, 328, andport 330 that, for example, pair with ports 310, 312, 324, 326,respectively. The ports 318, 320, 328, 330 can be suitably configured toreceive fluid and transport fluid. Port 320 can be offset from port 318relative to axis 314. Port 328 can be offset from port 330 relative toaxis 314. Port 318 can be positioned relative to axis 314 at the sameposition as port 328 or offset from port 328 relative to axis 314. Port320 can be positioned relative to axis 314 at the same position as port330 or offset from port 330 relative to axis 314. A distance port 320 isoffset from port 318 relative to axis 314 can be the same as a distanceport 312 is offset from port 310 relative to axis 306 so that there is apairing between ports 310 and 318, and ports 312 and 320 when the secondbody 304 is received by first body 302. A distance port 330 is offsetfrom port 328 relative to axis 314 can be the same as a distance port326 is offset from port 324 relative to axis 306 so that there is apairing between ports 324 and 328, and ports 326 and 330 when the secondbody 304 is received by first body 302.

The treatment indicator 122 can be positioned within the chamber 316.The second body 304 can comprise a shape configured to be received bythe cavity 308 of the first body 302. For example, the second body 304can be positioned within the cavity 308 of the first body 302. Thesecond body 304 can be moveable with respect to the first body 302and/or the first body 302 can be moveable with respect to the secondbody 304. For example, as illustrated in FIG. 4A, the article 300 can beconfigured in a first configuration. In the first configuration, port310 can be aligned with port 318 to form a first fluid pathway 350 tothe chamber 316; port 312 can be aligned with port 320 to form a secondfluid pathway 360 to the chamber 316; port 324 can be aligned with port328 to form a third fluid pathway 370 to the chamber 316; and port 326can be aligned with port 330 to form a fourth fluid pathway 380 to thechamber 316.

The article 300 can be configured to move between the firstconfiguration in FIG. 4A and a second configuration as illustrated inFIG. 4B. In the second configuration, port 310 can be misaligned withport 318; port 312 can be misaligned with port 320; port 324 can bemisaligned with port 328; and port 326 can be misaligned with port 330.The misalignment can inhibit or prevent traversal of fluid through atleast one of the first fluid pathway 350, the second fluid pathway 360,the third fluid pathway 370, and the fourth fluid pathway 380 via, forexample, the full or partial blockage of ports 310, 312, 318, 320, 324,326, 328, 330. For example, in the second configuration of the article300, the chamber 316 can be a closed system and fluid within the chamber316 can be inhibited or prevented from exiting the chamber 316.

The article 300 can comprise a reservoir 332 configured to retain growthmedia. The reservoir 332 can be positioned within the chamber 316, andthe reservoir 332 can comprise a seal 334 configured to retain growthmedia separate from the treatment indicator 122. The seal 334 cancomprise at least one of a film, a membrane, a wall, and a vial. Theseal 334 can comprise various materials, such as, for example, metallicmaterials, polymeric materials (e.g., plastic), ceramic materials, andglass materials.

The reservoir 332 can be configured to introduce the growth media to thetreatment indicator 122 upon degradation of the seal 334. For example,in the first configuration of the article 300 (FIG. 4A), the seal 334 ofthe reservoir 332 may be configured to retain the growth media. In thesecond configuration of the article 300 (FIG. 4B), the seal 334 of thereservoir 332 can be configured to break and to introduce the growthmedia to the treatment indicator 122. For example, the seal 334 can bepierced, punctured, snapped, and/or torn.

As illustrated in FIGS. 4A-B, the reservoir 332 can be integral to thesecond body 304 and the article 300 can comprise a seal disruptionmember 336 comprising a first end 336 a and a second end 336 b. Thefirst end 336 can be any shape or configuration that assists in breakingthe seal 334, such as, for example, one or more fork tangs, asillustrated, to pierce the seal 334 in one or more locations. In thefirst configuration of the article 300, the seal 334 can be positioned adistance, di, from the second end 336 b of the seal disruption member336. When changing the article 300 from the first configuration to thesecond configuration, the first end 336 a of the seal disruption member336 can engage the second end 304 b of the second body 304 and preventmovement of the seal disruption member 336 relative to axis 314 towardsthe second end 304 bof the second body 304. During the configurationchange of the article 300 from the first configuration to the secondconfiguration, the distance between the seal 334 and the second end 304b of the body 304 can decrease, thereby allowing the first end 336 a ofthe seal disruption member 336 to break the seal 334 and release growthmedia from the reservoir 332. The growth media can traverse into thechamber 316 and be introduced to and contact the treatment indicator122. In the second configuration of the article 300, the seal 334 can bepositioned a distance, d₂, from the second end 336 b of the sealdisruption member 336.

The reservoir 332 may not be integral to the second body 304 and may bea separate entity within the chamber 316. For example, the reservoir 332may be a third body positioned within the chamber 316. The seal 334 canbe a wall of the reservoir 332, which can be broken by changing thearticle 300 from the first configuration to the second configurationand/or another physical force. For example, seal 334 can be deformedcausing the seal 334 to break and release the growth media into thechamber 316. The reservoir 332 can comprise a seal 334 of a glass vial,and deformation of the glass vial breaks the glass vial and releases thegrowth media into the chamber 316.

The reservoir 332 may be positioned outside of the article 300 and incommunication with at least one of the ports, 310, 312, 324, 326. Thegrowth media can be released from the reservoir 332 into the chamber 316while the article 300 is in the first configuration, and then thearticle 300 can be positioned in the second configuration. It iscontemplated that growth media can be injected into the chamber 316 withan injection device, such as, for example, a syringe.

The article 300 can comprise a keyway 338 positioned on an end of thesecond body 304, such as, for example, the second end 304 b. The keyway338 can be configured to facilitate alignment of the article 300 with anapparatus for treatment and/or a detector.

In order to facilitate a measurement of the electromagnetic property,the first body 302 can comprise an opening 340 and the second body 304can comprise a corresponding transparent section 304 c. The transparentsection 304 c can be configured to allow transmission of electromagneticradiation into and/or through the chamber 316. For example, thetransparent section 304 c can comprise a material that minimallyabsorbs, or does not absorb, electromagnetic radiation that is emittedthrough the chamber 316. The opening 340 can be configured to align withthe transparent section 304 c of the second body 304 when the article300 is in a second configuration as illustrated in FIG. 4B. The firstbody 302 may be configured with a second transparent section (not shown)in place of the opening 340 and the second transparent section can beconfigured to transmit electromagnetic radiation into the transparentsection 304 c through the chamber 316. The transmission ofelectromagnetic radiation into and/or through the chamber 316 can enablemeasurement of an electromagnetic property of the treatment indicator122 within the chamber 316 and/or fluid within the chamber 316.

As illustrated in FIG. 5, the article 300 for indication of treatmentcan be introduced to a detector 542. The detector 542 can be configuredto measure an electromagnetic property of fluid within the chamber 316via the transparent section 304 c of the second body 304. The detector542 can comprise an electromagnetic radiation source 542 a suitable toemit electromagnetic radiation 544 a into the chamber 316 and anelectromagnetic radiation detector 542 b suitable to receive and measureelectromagnetic radiation 544 b. The electromagnetic radiation 544 aemitted by the electromagnetic radiation source 542 a can comprise awavelength in a wavelength range of 100 nm to 1000 nm, and theelectromagnetic radiation 544 b received and measured by theelectromagnetic radiation detector 542 b can comprise a wavelength in awavelength range of 100 nm to 1000 nm.

In examples where the electromagnetic property of the fluid comprisesfluorescence, the electromagnetic radiation 544 a can enter the chamber316 and interact with the fluid within the chamber 316. The interactioncan produce fluorescence as electromagnetic radiation 544 b, which canbe measured by electromagnetic radiation detector 542 b. In exampleswhere the electromagnetic property of the fluid comprises absorbance,the electromagnetic radiation 544 a can enter the chamber 316, and atleast part of the electromagnetic radiation 544 a can be absorbed by thefluid within the chamber 316. The remaining portion of electromagneticradiation 544 b can leave the chamber 316 as electromagnetic radiation544 b, which can be measured by electromagnetic radiation detector 542b.

FIG. 6 illustrates a system 600 comprising an apparatus 646 fortreatment and the article 300 for indication of treatment. Asillustrated, the apparatus 646 can comprise a chamber 648 including atreatment basin 650 in fluid communication with a reservoir 656. Thetreatment basin 650 can be configured to receive a device 652 to betreated. The chamber 648 can be suitably configured to perform atreatment process on the device 652 in the treatment basin 650. Thechamber 648 can comprise at least one of a heater (e.g., heatingelement), a pump, a wash arm, a spray nozzle, a tube, and other featuresknown to one of ordinary skill in the art. The chamber 648 can be atleast one of a cleaning chamber, a disinfection chamber, and asterilization chamber. The device 652 can be, for example, a medicaldevice such as, for example, an endoscope. The apparatus 646 cancomprise an endoscope re-processor (e.g., an automated endoscopere-processor).

The chamber 648 can comprise a vessel 654 positioned separate from thetreatment basin 650. The vessel 654 can be in fluid communication withat least one of the treatment basin 650 and the reservoir 656. Thevessel 654 can comprise a cavity suitable to receive the article 300 forindication of treatment. The vessel 654 can be configured to provide afluid to the article 300, such as, for example, growth media, a rinseagent, and a treatment agent. The vessel 654 can subject the article 300to the same treatment process that the device 652 is subjected to in thebasin 650 such that the article 300 can accurately represent the degreeto which the device 652 is treated.

The present disclosure provides a method for indication of treatment.The method comprises introducing the article 300 for indication oftreatment according to the present disclosure into an apparatus 646 fortreatment. The article 300 for indication of treatment can be introducedinto a treatment basin 650 of the apparatus 646 or a vessel positionedseparate from the treatment basin. A device 652 to be treated can beintroduced into the treatment basin 650 of the apparatus 646.

The device 652 and article 300 can be subjected to a treatment process.The treatment process can comprise introducing a treatment solution tothe device 652. Concomitantly, the treatment solution can be introducedto a chamber of the article for indication of treatment according to thepresent disclosure. The treatment solution can contact a treatmentindicator 122 in the article, as provided here. Responsive to contactand if active, the treatment solution can reduce and/or eliminatebiological material on the treatment indicator 122. The treatmentsolution can be removed from the chamber of the article 300 responsiveto removal of the treatment solution from the device 652. The article300 for indication of treatment according to the present disclosure canbe used to measure the effectiveness of the treatment process. Thearticle 300 for indication of treatment according to the presentdisclosure can be removed from the apparatus 646 for treatment or leftin the apparatus 646 for treatment.

The article 300 can be changed from the first configuration to thesecond configuration, as set forth herein, and can introduce and contactthe treatment indicator 122 with the growth media. The growth media canbe incubated with the treatment indicator in order to determine theeffectiveness of the treatment process. An electromagnetic property ofat least one of the growth media and treatment indicator can bemeasured.

One skilled in the art will recognize that the herein describedcomponents (e.g., operations), devices, objects, and the discussionaccompanying them are used as examples for the sake of conceptualclarity and that various configuration modifications are contemplated.Consequently, as used herein, the specific exemplars set forth and theaccompanying discussion are intended to be representative of their moregeneral classes. In general, use of any specific exemplar is intended tobe representative of its class, and the non-inclusion of specificcomponents (e.g., operations), devices, and objects should not be takenas limiting.

The herein described subject matter sometimes illustrates differentcomponents contained within, or connected with, other differentcomponents. It is to be understood that such depicted architectures aremerely exemplary, and that in fact many other architectures may beimplemented that achieve the same functionality. In a conceptual sense,any arrangement of components to achieve the same functionality iseffectively “associated” such that the desired functionality isachieved. Hence, any two components herein combined to achieve aparticular functionality can be seen as “associated with” each othersuch that the desired functionality is achieved irrespective ofarchitectures or intermedial components. Likewise, any two components soassociated can also be viewed as being “operably connected” or “operablycoupled” to each other to achieve the desired functionality, and any twocomponents capable of being so associated can also be viewed as being“operably couplable” to each other to achieve the desired functionality.Specific examples of operably couplable include, but are not limited to,physically mateable and/or physically interacting components, and/orwirelessly interactable, and/or wirelessly interacting components,and/or logically interacting, and/or logically interactable components.

With respect to the appended claims, those skilled in the art willappreciate that recited operations therein may generally be performed inany order. Also, although various operational flows are presented in asequence(s), it should be understood that the various operations may beperformed in other orders than those which are illustrated or may beperformed concurrently. Examples of such alternate orderings may includeoverlapping, interleaved, interrupted, reordered, incremental,preparatory, supplemental, simultaneous, reverse, or other variantorderings, unless context dictates otherwise. Furthermore, terms like“responsive to,” “related to,” or other past-tense adjectives aregenerally not intended to exclude such variants, unless context dictatesotherwise.

Although various examples have been described herein, manymodifications, variations, substitutions, changes, and equivalents tothose examples may be implemented and will occur to those skilled in theart. Also, where materials are disclosed for certain components, othermaterials may be used. It is therefore to be understood that theforegoing description and the appended claims are intended to cover allsuch modifications and variations as falling within the scope of thedisclosed examples. The following claims are intended to cover all suchmodification and variations.

Any patent, publication, or other disclosure material, in whole or inpart, that is said to be incorporated by reference herein isincorporated herein only to the extent that the incorporated materialsdoes not conflict with existing definitions, statements, or otherdisclosure material set forth in this disclosure. As such, and to theextent necessary, the disclosure as explicitly set forth hereinsupersedes any conflicting material incorporated herein by reference.Any material, or portion thereof, that is said to be incorporated byreference herein, but which conflicts with existing definitions,statements, or other disclosure material set forth herein will only beincorporated to the extent that no conflict arises between thatincorporated material and the existing disclosure material.

Various examples according to the present disclosure include, but arenot limited to, the examples listed in the following numbered clauses.

-   -   1. An article for indication of treatment comprising a first        body comprising: a first axis; a cavity positioned within the        first body and configured to receive a second body; and a first        port and a second port, the second port offset from the first        port relative to the first axis. The second body comprising: a        second axis; a chamber positioned within the second body and        configured to receive a fluid; and a third port and a fourth        port, the fourth port offset from the third port relative to the        second axis. Wherein the article is configured to move between a        first configuration and a second configuration. Wherein in the        first configuration the first port is aligned with the third        port to form a fluid pathway to the chamber and the second port        is aligned with the fourth port to form a fluid pathway to the        chamber. Wherein in the second configuration the first port is        misaligned with the third port and the second port is misaligned        with the fourth port. A treatment indicator is positioned within        the chamber.    -   2. The article of clause 1, further comprising a reservoir        positioned within the chamber, the reservoir comprising a seal        configured to retain growth media separate from the treatment        indicator.    -   3. The article of clause 2, wherein the seal is configured to        break responsive to the second configuration of the article, the        breaking of the seal suitable to introduce the growth media to        the treatment indicator.    -   4. The article of any one of clause 2-3, wherein the seal        comprises at least one of a film, a membrane, a wall, and a        vial.    -   5. The article of any one of clauses 1-4, wherein the treatment        indicator comprises biological material.    -   6. The article of clause 5, wherein the biological material        comprises at least one of an archaeon, an eukaryote, a        bacterium, and a virus.    -   7. The article of clause 6, wherein the biological material        comprises a bacterial spore.    -   8. The article of any one of clauses 5-7, wherein the biological        material degrades responsive to at least one of a disinfectant        and a sterilant.    -   9. The article of any one of clauses 1-8, wherein the first        configuration of the article is suitable to gravity drain fluid        from the chamber.

10. The article of any one of clauses 1-9, wherein the article isconfigured to rotate at least one of the first body around the firstaxis and the second body around the second axis to change between thefirst and second configuration.

-   -   11. The article of any one of clauses 1-10, wherein the article        is configured to translate at least one of the first body        relative to the first axis and the second body relative to the        second axis to change between the first and second        configuration.    -   12. The article of any one of clauses 1-11, further comprising a        keyway positioned on an end of the second body.    -   13. The article of any one of clauses 1-12, wherein the second        body comprises a transparent section configured to transmit        electromagnetic radiation through the chamber.    -   14. The article of clause 13, further comprising an opening in        the first body, the opening configured to align with the        transparent section of the second body when the article is in        the second configuration.    -   15. The article of any one of clauses 1-14, further comprising        at least one of a spring and a piston configured to move the        article between the first configuration and the second        configuration.    -   16. A system comprising: an article for indication of a        treatment comprising a first body comprising: a first axis; a        cavity positioned within the first body and configured to        receive a second body; and a first port and a second port, the        second port offset from the first port relative to the first        axis. The second body comprising: a second axis; a chamber        positioned within the second body and configured to receive a        fluid; a third port and a fourth port, the fourth port offset        from the third port relative to the second axis; and a        transparent section configured to transmit electromagnetic        radiation through the chamber. Wherein the article is configured        to move between a first configuration and a second        configuration. Wherein in the first configuration the first port        is aligned with the third port to form a fluid pathway to the        chamber and the second port is aligned with the fourth port to        form a fluid pathway to the chamber. Wherein in the second        configuration the first port is misaligned with the third port        and the second port is misaligned with the fourth port. A        treatment indicator is positioned within the chamber. A detector        is configured to measure an electromagnetic property of fluid        within the chamber via the transparent section of the second        body.    -   17. The system of clause 16, wherein the electromagnetic        property is at least one of absorption, transmittance,        scattering, reflectance, and photoluminescence.    -   18. The system of any one of clauses 16-17, wherein the        electromagnetic property is measured in a wavelength range of        100 nm to 1000 nm.    -   19. A system comprising an apparatus for treatment, the        apparatus for treatment comprising: a treatment basin configured        to receive a device to be treated; and a vessel positioned        separate from the treatment basin. The vessel is in fluid        communication with the treatment basin and suitable to receive        an article for indication of treatment. The vessel is configured        to provide a treatment agent to the article. Wherein the article        comprises a first body comprising: a first axis; a cavity        positioned within the first body and configured to receive a        second body; and a first port and a second port, the second port        offset from the first port relative to the first axis. The        second body comprising: a second axis; a chamber positioned        within the second body and configured to receive a fluid; and a        third port and a fourth port, the fourth port offset from the        third port relative to the second axis. Wherein the article is        configured to move between a first configuration and a second        configuration. Wherein in the first configuration the first port        is aligned with the third port to form a fluid pathway to the        chamber and the second port is aligned with the fourth port to        form a fluid pathway to the chamber. Wherein in the second        configuration the first port is misaligned with the third port        and the second port is misaligned with the fourth port. A        treatment indicator is positioned within the chamber.    -   20. An article for indication of treatment comprising a first        body comprising: a first axis; a cavity positioned within the        first body and configured to receive a second body; and a first        port and a second port, the second port offset from the first        port relative to the first axis. The second body comprising: a        second axis; a chamber positioned within the second body and        configured to receive a fluid; a transparent section configured        to transport electromagnetic radiation through the chamber; and        a third port and a fourth port, the fourth port offset from the        third port relative to the second axis. Wherein the article is        configured to move between a first configuration and a second        configuration. Wherein in the first configuration the first port        is aligned with the third port to form a fluid pathway to the        chamber and the second port is aligned with the fourth port to        form a fluid pathway to the chamber. Wherein in the second        configuration the first port is misaligned with the third port        and the second port is misaligned with the fourth port. A        treatment indicator is positioned within the chamber and        comprising biological material. A reservoir is positioned within        the chamber. The reservoir comprising a seal configured to        retain growth media separate from the treatment indicator. The        seal is configured to break responsive to the second        configuration of the article. The breaking of the seal suitable        to introduce the growth media to the treatment indicator.    -   21. A method for indication of treatment, the method comprising:        introducing the article of clause 20 into an apparatus for        treatment; introducing a treatment solution into the chamber of        the article in the first configuration; removing the treatment        solution from the chamber; changing the article from the first        configuration to the second configuration to contact the        treatment indicator with the growth media; and measuring an        electromagnetic property of at least one of the growth media and        treatment indicator.    -   22. The method of clause 21, further comprising incubating the        growth media with the treatment indicator.    -   23. The method of any one of clauses 21-22, wherein the        biological material comprises at least one of an archaeon, an        eukaryote, a bacterium, and a virus.    -   24. The method of clause 23, wherein the biological material        comprises bacterial spores.    -   25. The method of any one of clauses 21-24, further comprising:        introducing a device to be treated into a treatment basin of the        apparatus for treatment; and introducing the article into a        vessel positioned separate from the treatment basin.    -   26. The method of any one of clauses 21-25, wherein the        electromagnetic property is at least one of absorption,        transmittance, scattering, reflectance, and photoluminescence.    -   27. The method of any one of clauses 21-26, wherein the        electromagnetic property is measured at a wavelength in a range        of 100 nm to 1000 nm.    -   28. The method of any one of clauses 21-27, wherein changing the        article from the first configuration to the second configuration        is manual or automatic.

Numerous benefits have been described which result from employing theconcepts described herein. The foregoing description of the one or moreexamples has been presented for purposes of illustration anddescription. It is not intended to be exhaustive or limiting to theprecise form disclosed. Modifications or variations are possible inlight of the above teachings. The one or more examples were chosen anddescribed in order to illustrate principles and practical application tothereby enable one of ordinary skill in the art to utilize the variousexamples and with various modifications as are suited to the particularuse contemplated. It is intended that the claims submitted herewithdefine the overall scope.

While the present disclosure provides descriptions of various specificexamples for the purpose of illustrating various examples of the presentdisclosure and/or its potential applications, it is understood thatvariations and modifications will occur to those skilled in the art.Accordingly, the invention or inventions described herein should beunderstood to be at least as broad as they are claimed, and not as morenarrowly defined by particular illustrative examples provided herein.

What is claimed is:
 1. An article for indication of treatmentcomprising: a first body comprising: a first axis; a cavity positionedwithin the first body and configured to receive a second body; and afirst port and a second port, the second port offset from the first portrelative to the first axis; the second body comprising: a second axis; achamber positioned within the second body and configured to receive afluid; and a third port and a fourth port, the fourth port offset fromthe third port relative to the second axis; wherein the article isconfigured to move between a first configuration and a secondconfiguration, wherein in the first configuration the first port isaligned with the third port to form a fluid pathway to the chamber andthe second port is aligned with the fourth port to form a fluid pathwayto the chamber, and wherein in the second configuration the first portis misaligned with the third port and the second port is misaligned withthe fourth port; and a treatment indicator positioned within thechamber.
 2. The article of claim 1, further comprising a reservoirpositioned within the chamber, the reservoir comprising a sealconfigured to retain growth media separate from the treatment indicator.3. The article of claim 2, wherein the seal is configured to breakresponsive to the second configuration of the article, the breaking ofthe seal suitable to introduce the growth media to the treatmentindicator.
 4. The article of claim 2, wherein the seal comprises atleast one of a film, a membrane, a wall, and a vial.
 5. The article ofclaim 1, wherein the treatment indicator comprises biological material.6. The article of claim 5, wherein the biological material comprises atleast one of an archaeon, an eukaryote, a bacterium, and a virus.
 7. Thearticle of claim 6, wherein the biological material comprises abacterial spore.
 8. The article of claim 5, wherein the biologicalmaterial degrades responsive to at least one of a disinfectant and asterilant.
 9. The article of claim 1, wherein the first configuration ofthe article is suitable to gravity drain fluid from the chamber.
 10. Thearticle of claim 1, wherein the article is configured to rotate at leastone of the first body around the first axis and the second body aroundthe second axis to change between the first and second configuration.11. The article of claim 1, wherein the article is configured totranslate at least one of the first body relative to the first axis andthe second body relative to the second axis to change between the firstand second configuration.
 12. The article of claim 1, further comprisinga keyway positioned on an end of the second body.
 13. The article ofclaim 1, wherein the second body comprises a transparent sectionconfigured to transmit electromagnetic radiation through the chamber.14. The article of claim 13, further comprising an opening in the firstbody, the opening configured to align with the transparent section ofthe second body when the article is in the second configuration.
 15. Thearticle of claim 1, further comprising at least one of a spring and apiston configured to move the article between the first configurationand the second configuration.
 16. A system comprising: the article ofclaim 1, wherein the second body further comprises a transparent sectionconfigured to transmit electromagnetic radiation through the chamber;and a detector configured to measure an electromagnetic property offluid within the chamber via the transparent section of the second body.17. The system of claim 16, wherein the electromagnetic property is atleast one of absorption, transmittance, scattering, reflectance, andphotoluminescence.
 18. The system of claim 16, wherein theelectromagnetic property is measured in a wavelength range of 100 nm to1000 nm.
 19. A system comprising: an apparatus for treatment, theapparatus for treatment comprising: a treatment basin configured toreceive a device to be treated; and a vessel positioned separate fromthe treatment basin, the vessel in fluid communication with thetreatment basin and suitable to receive an article for indication oftreatment, the vessel configured to provide a treatment agent to thearticle; wherein the article comprises: a first body comprising: a firstaxis; a cavity positioned within the first body and configured toreceive a second body; and a first port and a second port, the secondport offset from the first port relative to the first axis; the secondbody comprising: a second axis; a chamber positioned within the secondbody and configured to receive a fluid; and a third port and a fourthport, the fourth port offset from the third port relative to the secondaxis; wherein the article is configured to move between a firstconfiguration and a second configuration, wherein in the firstconfiguration the first port is aligned with the third port to form afluid pathway to the chamber and the second port is aligned with thefourth port to form a fluid pathway to the chamber, and wherein in thesecond configuration the first port is misaligned with the third portand the second port is misaligned with the fourth port; and a treatmentindicator positioned within the chamber.
 20. An article for indicationof treatment comprising: a first body comprising: a first axis; a cavitypositioned within the first body and configured to receive a secondbody; and a first port and a second port, the second port offset fromthe first port relative to the first axis; the second body comprising: asecond axis; a chamber positioned within the second body and configuredto receive a fluid; a transparent section configured to transportelectromagnetic radiation through the chamber; and a third port and afourth port, the fourth port offset from the third port relative to thesecond axis; wherein the article is configured to move between a firstconfiguration and a second configuration, wherein in the firstconfiguration the first port is aligned with the third port to form afluid pathway to the chamber and the second port is aligned with thefourth port to form a fluid pathway to the chamber, and wherein in thesecond configuration the first port is misaligned with the third portand the second port is misaligned with the fourth port; a treatmentindicator positioned within the chamber and comprising biologicalmaterial; and a reservoir positioned within the chamber, the reservoircomprising a seal configured to retain growth media separate from thetreatment indicator, the seal is configured to break responsive to thesecond configuration of the article, the breaking of the seal suitableto introduce the growth media to the treatment indicator.
 21. A methodfor indication of treatment, the method comprising: introducing thearticle of claim 20 into an apparatus for treatment; introducing atreatment solution into the chamber of the article in the firstconfiguration; removing the treatment solution from the chamber;changing the article from the first configuration to the secondconfiguration to contact the treatment indicator with the growth media;and measuring an electromagnetic property of at least one of the growthmedia and treatment indicator.
 22. The method of claim 21, furthercomprising incubating the growth media with the treatment indicator. 23.The method of claim 21, wherein the biological material comprises atleast one of an archaeon, an eukaryote, a bacterium, and a virus. 24.The method of claim 23, wherein the biological material comprisesbacterial spores.
 25. The method of claim 21, further comprising:introducing a device to be treated into a treatment basin of theapparatus for treatment; and introducing the article into a vesselpositioned separate from the treatment basin.
 26. The method of claim21, wherein the electromagnetic property is at least one of absorption,transmittance, scattering, reflectance, and photoluminescence.
 27. Themethod of claim 21, wherein the electromagnetic property is measured ata wavelength in a range of 100 nm to 1000 nm.
 28. The method of claim21, wherein changing the article from the first configuration to thesecond configuration is manual or automatic.